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Shamier Little is the daughter of Sean Little and Tiffany Mayfield. She was born on March 20, 1995 in Louisville, Kentucky and has three siblings: Timothy, Shakira, and Samiyre. Her mother competed in track and field at Eastern Kentucky and cleared 5-8 in high jump. Little started competing in track and field at age eight. She competed in the 2004 USATF Youth Championships in the 100m dash and won the 2012, 2014, and 2015 USATF Junior Championships in the 400m hurdles. In college, Little started out as an agricultural leadership major but switched to business management. She wanted to become a firefighter after the end of her athletic career.